鎮ㄧ従(xi脿n)鍦ㄧ殑浣嶇疆锛�棣栭爜 > 鍎€鍣ㄥぇ鍏� > 妞嶇墿鐢熺悊鐢熸厠(t脿i)鍎€鍣� > 杞�(zhu菐n)鍩哄洜妾㈡脯(c猫)瑭︾礄 > 杞�(zhu菐n)鍩哄洜娓�(c猫)瑭︾洅
  • 杞�(zhu菐n)鍩哄洜娓�(c猫)瑭︾洅
  • 杞�(zhu菐n)鍩哄洜娓�(c猫)瑭︾洅
  • 杞�(zhu菐n)鍩哄洜娓�(c猫)瑭︾洅
  • 杞�(zhu菐n)鍩哄洜娓�(c猫)瑭︾洅

杞�(zhu菐n)鍩哄洜娓�(c猫)瑭︾洅

鐢�(ch菐n)鍝佸瀷铏�(h脿o)锛欵PSPSCP4
鐢�(ch菐n)鍝佸児(ji脿)鏍硷細鍦ㄧ窔瑭㈠児(ji脿)

杞�(zhu菐n)鍩哄洜娓�(c猫)瑭︾洅/EPSPSCP4杞�(zhu菐n)鍩哄洜瑭﹀姂鐩�鏄敤鐗圭暟鎬ф姉 Cry1Ab/1Ac 鍠姉鍖呰鐨勫井瀛旀澘鍜岄叾妯�(bi膩o)瑷樻姉 Cry1Ab/1Ac 鍠姉锛岄洐鎶楅珨澶惧績娉曟娓�(c猫) Cry1Ab/1Ac 鍩哄洜琛ㄩ仈(d谩)鐢�(ch菐n)鐗�锛屽彲浠ラ珮闈堟晱搴﹀拰鐗圭暟鎬х殑妾㈡脯(c猫)杞�(zhu菐n)鍩哄洜妞嶇墿涓殑 Bt-Cry1Ab/1Ac 铔嬬櫧銆�

  • 鍦ㄧ窔瀹㈡湇锛�
  • 鍏嶈不(f猫i)鍜ㄨ鐔辩窔锛�0571-86056609 86059660 88971438

鐢�(ch菐n)鍝佽┏鎯�

    銆� 瑭﹀姂鐩掑師鐞嗐€�
        鏈搧绯荤敤鐗圭暟鎬ф姉 Cry1Ab/1Ac 鍠姉鍖呰鐨勫井瀛旀澘鍜岄叾妯�(bi膩o)瑷樻姉 Cry1Ab/1Ac 鍠姉锛岄洐鎶楅珨澶惧績娉曟娓�(c猫) Cry1Ab/1Ac 鍩哄洜琛ㄩ仈(d谩)鐢�(ch菐n)鐗�锛屽彲浠ラ珮闈堟晱搴﹀拰鐗圭暟鎬х殑妾㈡脯(c猫)杞�(zhu菐n)鍩哄洜妞嶇墿涓殑 Bt-Cry1Ab/1Ac 铔嬬櫧銆�

     

    銆愯│鍔戠洅绲勪唤锛堣(gu墨)鏍硷級銆�
    闋�(y霉)鍖呰鏉匡紙鎶� Cry1Ab/1Ac 鍠姉锛�      12 瀛�×8
    Cry1Ab/1Ac 閰舵(bi膩o)鎶楅珨                 12 ml×1 鐡�
    20×婵冪府娲楁粚娑�                      30 ml×1 鐡�
    5×鎶芥彁娑�/绋€閲嬬珐娌栨恫                30 ml×1 鐡�
    搴曠墿娑� A                            7 ml×1 鐡�
    搴曠墿娑� B                            7 ml×1 鐡�
    绲傛娑�                              7 ml×1 鐡�
    浣跨敤瑾槑鏇�                          1 浠�
    0ppb 闄版€у皪(du矛)鐓�                       1ml
    4 鍊�(g猫)涓嶅悓婵冨害鐨勬(bi膩o)妯o細           
    0.5 ppb 1.0 ppb 2.0 ppb 4.0 ppb          鍚� 1ml

     

    銆愯│鍔戞簴(zh菙n)鍌欍€�
        瑭﹀姂鐩掍腑鐨� 20×婵冪府娲楁粚娑� 30 ml 鍔� 570 ml 婊呰弻 ddH2O 婧惰В绋€閲嬭嚦 1×娲楁粚娑�锛�5×鎶芥彁娑诧紙30 ml锛夊姞 120 ml ddH2O 绋€閲嬭嚦 1×鎶芥彁娑诧紝4鈩冨瓨鏀惧倷鐢�銆�


    銆愭ǎ鍝佹簴(zh菙n)鍌欍€�
        绋卞彇绱� 20 mg 钁夌墖锛屽姞 0.5 ml 1×鎶芥彁娑诧紙浣跨敤鍓� 30 min 鏀剧疆瀹ゆ韩锛夌爺纾ㄨ嚦鍕绘伎锛屽婧斁缃� 30 min锛屾脯(c猫)瀹氬墠鎶婃ǎ鍝佷笂娓呮恫绋€閲� 5-20 鍊�銆�
        娓�(c猫)瀹氳帠骞插拰鑳氫钩涓殑 Bt 铔嬬櫧鏅�(sh铆)锛屾ǎ鍝佺ū鍙栫磩 40 mg锛屽姞 1 ml 1×鎶芥彁娑茬爺纾�銆�

    銆怑LISA 鍙嶆噳(y墨ng)銆�
    1. 渚濇鍔� 100 μl 闄版€у皪(du矛)鐓�锛屾(bi膩o)婧�(zh菙n)鍝侊紙4 鍊�(g猫)涓嶅悓婵冨害鐨勬(bi膩o)妯o細0.5 ppb锛�1.0 ppb锛�2.0 ppb, 4.0 ppb锛夊拰寰呮脯(c猫)妯e搧鑷� 96 瀛旀澘锛�
    2. 鐢ㄥ皝鍙h啘钃嬩綇 96 瀛旀澘锛屽揩閫熸悥鍕�(d貌ng)骞虫澘娣峰嫽妯e搧锛�
    3. 37鈩冩亽婧瀛佃偛 30 min锛�
    4. 鍊掓帀 96 瀛旀澘涓恫楂旓紝姣忓瓟鍔犲叆 1×娲楁粚娑� 250 μl 娲楁粚寰瓟鏉�锛屾礂鏉� 5 娆★紝鐢ㄥ惛姘寸礄鎷嶅共锛�
    5. 鎸夐爢搴忓湪姣忓€�(g猫)灏忓瓟涓姞鍏� 100 μl Cry1Ab/1Ac 閰舵(bi膩o)鎶楅珨锛岀劧鍚庨噸寰�(f霉)姝ラ 2锛�
    6. 37 鈩冩亽婧瀛佃偛 30 min锛�
    7. 鍊掓帀 96 瀛旀澘涓恫楂�锛屾瘡瀛斿姞鍏� 1×娲楁粚娑� 250 μl 娲楁粚寰瓟鏉匡紝娲楁澘 5 娆�锛岀敤鍚告按绱欐媿骞�锛�
    8. 渚濇鍔犲叆 50 μl 搴曠墿 A 娑�锛�50 μl 搴曠墿 B 娑�锛岄噸寰�(f霉)姝ラ 2锛�
    9. 瀹ゆ韩锛�20-25鈩冿級閬垮厜瀛佃偛 10 min锛屽姞 50 μl 绲傛娑�锛屽厖鍒嗘贩鍕荤祩姝㈠弽鎳�(y墨ng)锛�15min 浠ュ収(n猫i)鍦ㄩ叾妯�(bi膩o)鍎€涓婅畝鍙栫祼(ji茅)鏋溿€� 鍦� 450 nm 鐐轰富娉㈤暦锛�630 nm 鐐烘娉㈤暦锛堟垨鍠尝闀� 450 nm锛変笅锛岀敤绌虹櫧瀛旀牎闆�锛屽啀璁€鍙栧悇瀛� OD 鍊�銆�

     

    銆愯綁(zhu菐n)鍩哄洜娓�(c猫)瑭︾洅浣跨敤娉ㄦ剰浜嬮爡(xi脿ng)銆�
    1. 娓�(c猫)瀹氫腑闇€浣跨敤鐨勬墍鏈夎│鍔戝拰 96 瀛旀澘鎻愬墠 30 min 鏀剧疆瀹ゆ韩婧偛锛堝惁鍓囨渻(hu矛)褰遍熆瑭﹂(y脿n)婧�(zh菙n)纰烘€э級锛屾敞鎰�12 閫g蹇呴爤婧偛鍚庡啀寰炲钩鏉夸笂鍙栦笅锛屾湭浣跨敤瀹岀殑闋�(y霉)鍖呰鏉挎鎳�(y墨ng)缃簬鏈夊共鐕ュ姂鐨勫皝鍙h涓瘑灏佷繚瀛�锛�
    2. 瑭﹀姂浣跨敤鍓嶆噳(y墨ng)鍏呭垎鎼栧嫽锛�
    3. 鎼栧嫊(d貌ng)骞虫澘娣峰嫽鏅�(sh铆)娉ㄦ剰閬垮厤妯e搧涔嬮枔浜ゅ弶姹℃煋锛�
    4. 浣跨敤娲楁粚娑叉紓娲楁檪(sh铆)锛屾礂婊屾恫闇€瑕佸~婊挎瘡鍊�(g猫)灏忓瓟锛岄€�(j矛n)琛屽厖鍒嗘紓娲�锛�
    5. 鐮旂(濂界殑妯e搧濡傛灉涓嶈兘涓€娆″叏閮ㄦ脯(c猫)閲忥紝鍙互鏆檪(sh铆)鍦� 4 鈩冮伩鍏変繚瀛�锛屼絾鏄渶濂藉湪 24 h 鍏�(n猫i)娓�(c猫)瀹氾紱
    6. 绲�(ji茅)鏋滃垽璁€璜�(q菒ng)?ji膩n)璇滅;?y墨ng)绲傛鍚� 15 min 鍏�(n猫i)瀹屾垚銆�


    銆怋t 铔嬬櫧婵冨害瑷�(j矛)绠楀叕寮忋€�

    Bt 铔嬬櫧婵冨害锛�μg/g锛�=


     

    銆愬劜(ch菙)瀛樻浠跺強鏈夋晥鏈熴€�
    瑭﹀姂鐩掑劜(ch菙)瀛樹簬 2-8鈩冿紝鍒囧嬁鍑嶅瓨锛涙湭闁嬪皝瑭﹀姂鐩掓湁鏁堟湡 12 鍊�(g猫)鏈�锛岄枊灏佸悗 3 鍊�(g猫)鏈堝収(n猫i)鐢ㄥ畬銆傦紙瑕嬪寘瑁濈洅涓婃(bi膩o)绨芥湁鏁堟湡锛�

     

    銆愯綁(zhu菐n)鍩哄洜娓�(c猫)瑭︾洅鎶€琛�(sh霉)鍙冩暩(sh霉)銆�
    鏈搧閬╃敤浜庣帀绫炽€佹鑺�銆佸ぇ璞嗕互鍙婂叾鍚勭ó杈�(n贸ng)浣滅墿涓綁(zhu菐n)鍩哄洜鎴愬垎鐨勫揩閫熷畾閲忔脯(c猫)瑭�銆�


鐢�(ch菐n)鍝佺洰閷�


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